Over the past several decades, the principal types of cancer therapies have been chemotherapy, radiation therapy, and surgery. While there have been some successes in treating certain types of neoplasms, many tumor types are refractory to modern therapies. Currently, magnetically induced heat from magnetic nanoparticle hyperthermia is considered as an effective method to treat cancer cells. It is reported in previous articles that these nanoparticles will produce substantial heat that typically increases the tumor temperature, which can treat cancer cells, and another major potential of magnetic nanoparticles is the ability to combine heat and drug release for cancer treatment. However, insufficient AC induction heating power at a biological safe range of AC magnetic field and highly required biocompatibility of superparamagnetic nanoparticle hyperthermia agents have still remained as critical challenges for successful clinical hyperthermia applications. In this work, two different types nanoparticles (Fe₃O₄, Fe₂O₃) were modified by whey protein isolation (WPI), these bio-modified superparamagnetic nanoparticles (WPI-SNPs) display excellent stability in different conditions and have a spherical shape with a diameter around between 40nm-100nm. Doxorubicin (DOX) was then successfully loaded into WPI-SNPs with a desirable loading content of 12%. In vitro and in vivo magnetic hyperthermia studies using WPI-SNPs nanofluids are conducted to estimate bio-availability and bio-feasibility, and experimental results prove WPI-SNPs nanofluids which show promising hyperthermia effects to completely treat the tumors.